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NEBNext® Blunt/TA Ligase Master Mix – 50 reactions (copy)
- Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components.
- No thawing is necessary as it remains liquid during storage at -20°C.
- Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.
- Suitable for Adaptor Ligation.
- Compatible with several Oxford Nanopore Technologies® workflows, including some involving Covid-19 research.
Product Information
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.
The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20°C.* Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.
* Freezers vary in their actual internal temperature. Our testing demonstrates that the master mix is liquid at -20°C. Freeze-thaw testing at -70°C has confirmed that the performance is unchanged after 20 freeze/thaw cycles.
Advantages and Feature
- Vector construction
- Linker ligation
- Fragment assembly
- Library construction
- TA cloning